Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.

Flow Cytometric Measurement of Cell Organelle Autophagy

Flow Cytometric Analysis by Bromodeoxyuridine/DNA Assay of Cell Cycle Perturbation of Methotrexate-treated Mouse LI 210 Leukemia Cells1

The in vitro effects of methotrexate (MTX) on cell cycle progression and DNA synthesis of LI 210 leukemia cells were studied by the bromodeoxyuridine (BrdUrd)/DNA analysis technique. Low dose (10~8 M) MTX which slightly inhibits clonal replication of the cells, delays progress across the S phase, and treatment for 24 h results in a slight increase of the S-phase population. Much higher doses (I...

Flow cytometric analysis by bromodeoxyuridine/DNA assay of cell cycle perturbation of methotrexate-treated mouse L1210 leukemia cells.

The in vitro effects of methotrexate (MTX) on cell cycle progression and DNA synthesis of L1210 leukemia cells were studied by the bromodeoxyuridine (BrdUrd)/DNA analysis technique. Low dose (10(-8) M) MTX, which slightly inhibits clonal replication of the cells, delays progress across the S phase, and treatment for 24 h results in a slight increase of the S-phase population. Much higher doses ...

dna flow cytometric analysis in variable types of hydropic placentas

background: differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. objective: we analyzed the nuclear dna content of various types of hydropic placentas by flowcytometry. materials and methods: dna ploidy analysis was performed i...

Comparison of image analysis and flow cytometric determination of cellular DNA content.

A good correlation (r = 0.94) was obtained between the DNA indices (DI) using flow cytometry and image analysis of nuclei cytospins extracted from paraffin wax embedded tumour sections. Some of the limitations and problems associated with image analysis which came to light included an unacceptably high coefficient of variation (CV) and a "left-shift" in the DI in most DNA histograms obtained wh...